Use of bromodeoxyuridine immunocapture to identify psychrotolerant phenanthrene-degrading bacteria in phenanthrene-enriched polluted Baltic Sea sediments
نویسندگان
چکیده
منابع مشابه
Phenanthrene degradation in soils co-inoculated with phenanthrene-degrading and biosurfactant-producing bacteria.
Contaminant sorption within the soil matrix frequently limits biodegradation. However, contaminant bioavailability can be species-specific. This study investigated bioavailability of phenanthrene (PHE) to two PHE-degrading bacteria (Pseudomonas strain R and isolate P5-2) in the presence of rhamnolipid biosurfactant and/or a biosurfactant-producing bacterium, Pseudomonas aeruginosa ATCC 9027. Ps...
متن کاملBioremediation of Phenanthrene Polluted Soils
The best metabolic conditions for the biodegradation of hydrocarbons by a selected microbial consortium from a natural soil were investigated in order to improve the efficiency of bioremediation processes for cleaning of polluted waters and soils. Phenanthrene is one of the most recalcitrant pollutants to microbial decomposition and most difficult to remove. A microbial consortium was selected ...
متن کاملNovel Phenanthrene-Degrading Bacteria Identified by DNA-Stable Isotope Probing
Microorganisms responsible for the degradation of phenanthrene in a clean forest soil sample were identified by DNA-based stable isotope probing (SIP). The soil was artificially amended with either 12C- or 13C-labeled phenanthrene, and soil DNA was extracted on days 3, 6 and 9. Terminal restriction fragment length polymorphism (TRFLP) results revealed that the fragments of 219- and 241-bp in Ha...
متن کاملisolation, identification and optimization of phenanthrene degrading bacteria from the coastal sediments of nayband bay
materials and methods phe degrading bacteria were isolated from surface sediments. isolated strains were then identified by biochemical and molecular (16s rdna gene sequence) analysis. rsm was employed to evaluate the optimum biodegradation of phe by the most effective isolated strain. the investigated parameters included the temperature, inoculum sizes, ph, nh4cl concentration, and salinity. o...
متن کاملPhenanthrene-degrading phenotype of Alcaligenes faecalis AFK2.
A phenanthrene-degrading bacterium that assimilated a wide range of organic compounds was isolated from a soil sample and identified as Alcaligenes faecalis strain AFK2. The strain degraded phenanthrene through protocatechuate, but did not utilize naphthalene. The phenanthrene-degrading phenotype (Phn+) of AFK2 disappeared after 20 successive subcultures in a mineral salts medium containing o-p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 2008
ISSN: 0168-6496,1574-6941
DOI: 10.1111/j.1574-6941.2008.00513.x